Clinical Chemistry Blog Notes 16B

After the release of my 1st post here about the adrenal cortex and its hormones, I'm sure you've been so pretty curious a lot about how can we relate this to what we examine always here in Clinical Chemistry, which is serum.

Now, in this post, you will find some pretty cool information, on how they are tested and at the same time to be related to what particular disease your mom, your neighbor, your cousin, your friend, your community, your fellow citizen or you might be having.. Just kidding!!ahaha!!

But before we go to anything else and dig deeper with the test involved in our precious adrenal cortex, let's take a brief recall on some basic principle on our serology subject, that will help us be more understand the principle underlying this test.

Let's review what we have learned about the principle of the RIA or Radioimmunoassay.

As we commonly know, RIA makes use of radioactive isotopes in the test.

Then this isotopes will be coated to the hormone to be assayed. Then it will be allowed to chemically react to the antibody which is specific against it.

Then, a patient serum is added to the reaction. Hence, the patient serum will tend to battle against the hot antigen( antigen that first combined) for partnership with the antibody. As this reaction continues to precede, presence of free bound antigen become to be supernatant. And this is the one being measured for the antigen.

A portray below is given for easier understanding:

photo from:

After knowing the basics, now let's go to the real subject matter.